caga vaca s1 m1 strains  (ATCC)

Journal: Journal of extracellular vesicles

Article Title: Development of a Recombinant Outer Membrane Vesicles (OMVs)-Based Vaccine Against Helicobacter pylori Infection in Mice.

doi: 10.1002/jev2.70085

Figure Lengend Snippet: FIGURE 3 Construction, heterologous antigen protein characterisation, and quantities of genetically engineered OMVs. (A) A diagram showing the generation of OMVs expressing UreB, VacA and CagA alone or co-expressing these three antigens simultaneously using the Hbp autotransporter platform. In brief, the side domains d1 and d2 of the passenger domain were replaced by antigens UreB and VacA, respectively, and the CagA antigen was inserted into side domain d4. A cartoon showing Hbp-mediated UreB, VacA and CagA ligation to the surface of outer membrane vesicles. The protein structures were generated using PyMOL. (B) A list of the OMVs used in this study. (C) SDS-PAGE analysis showing that UreB, VacA and CagA were successfully introduced into the OMVs derived from the H. pylori ΔlpxE ΔlpxF ΔfutB mutant strain. The molecular weights of UreB, VacA and CagA proteins are located in the regions indicated by the protein markers of 55–70 kDa, 70–100 kDa and 35–40 kDa, respectively, as indicated by the red markers. (D) The western blot shows the protein bands that are consistent with the UreB antigenic protein in all strains, indicating its successful introduction into the corresponding mutant strain. Furthermore, different combinations of antigenic proteins UreB, VacA and CagA were introduced into OMVs, and their serum was collected 4 weeks after the final immunisation to detect antigen-specific IgG levels (E–G). The levels of anti-OMPs IgG and anti-inactivated whole cell IgG were also evaluated (H and I). All antibodies were measured by ELISA. The means were compared using the least significant difference test. *p < 0.05 and **p < 0.01 represent differences between relevant groups.

Article Snippet: The expression of different proteins in the OMVs was detected by immunoblotting using rabbit serum antibodies against UreB, VacA or CagA (UreB, Cat. Orb359898, Biorbyt; VacA, Cat. Orb51658, Biorbyt; CagA, Cat. Orb23539, Biorbyt).

Techniques: Expressing, Ligation, Membrane, Generated, SDS Page, Derivative Assay, Mutagenesis, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Journal of extracellular vesicles

Article Title: Development of a Recombinant Outer Membrane Vesicles (OMVs)-Based Vaccine Against Helicobacter pylori Infection in Mice.

doi: 10.1002/jev2.70085

Figure Lengend Snippet: FIGURE 4 Identification of mucosal immune response induced by OMVs delivering H. pylori antigens. Concentrations of anti-UreB, VacA and CagA secretory IgA (A, B and C), and anti-OMPs secretory IgA (D) and anti-inactivated whole cells secretory IgA (E) secreted by the gastric mucosa were measured by enzyme-linked immunosorbent assay (ELISA) at Week 8, respectively. The means were compared using the least significant difference test. *p < 0.05 and **p < 0.01 represent differences between relevant groups.

Article Snippet: The expression of different proteins in the OMVs was detected by immunoblotting using rabbit serum antibodies against UreB, VacA or CagA (UreB, Cat. Orb359898, Biorbyt; VacA, Cat. Orb51658, Biorbyt; CagA, Cat. Orb23539, Biorbyt).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of extracellular vesicles

Article Title: Development of a Recombinant Outer Membrane Vesicles (OMVs)-Based Vaccine Against Helicobacter pylori Infection in Mice.

doi: 10.1002/jev2.70085

Figure Lengend Snippet: FIGURE 7 A schematic diagram showing the mechanisms by which engineered OMVs present the H. pylori antigen proteins UreB, CagA and VacA as vaccines.

Article Snippet: The expression of different proteins in the OMVs was detected by immunoblotting using rabbit serum antibodies against UreB, VacA or CagA (UreB, Cat. Orb359898, Biorbyt; VacA, Cat. Orb51658, Biorbyt; CagA, Cat. Orb23539, Biorbyt).

Techniques: Vaccines

Journal: Expert Reviews in Molecular Medicine

Article Title: Developing a potent vaccine against Helicobacter pylori : critical considerations and challenges

doi: 10.1017/erm.2024.19

Figure Lengend Snippet: A summary of the primary Helicobacter pylori vaccines published in the literature, including their compositional properties and immune response data

Article Snippet: Immunogen virulence factors, including VacA and CagA, are generally targeted for H. pylori vaccination.

Techniques: Vaccines, Adjuvant, Animal Model, Plasmid Preparation, Sterility, Activation Assay, Infection